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Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways
doi: 10.1186/s13046-018-0908-y
Figure Lengend Snippet: COMP level is increased in the serum of HCC patients. a ELISA analysis of COMP serum level in 100 HCC patients and 30 healthy controls. P = 0.0115 by t test versus normal controls. b ROC curve of serum COMP in 100 HCC patients and 30 normal controls. c and d The overall survival and disease-free survival of HCC patients with high or low level of COMP estimated using the Kaplan-Meier analysis and compared by the Log-rank test in the same set of patients
Article Snippet: Quantitative measurement of human COMP in serum of HCC patients were performed by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways
doi: 10.1186/s13046-018-0908-y
Figure Lengend Snippet: LX2 cells-derived COMP drives tumor progression. a COMP concentrations (detected by ELISA) in conditioned media (CM) and COMP expression (detected by Western blot) in 5 HCC cell lines and hepatocytes LO2 and activated hepatic stellate cell LX2. LO2 was used as a negative control. n = three independent repeats. P < 0.05 by t test versus LO2. b The marker of activated hepatic stellate cells α-SMA was confirmed using IF. Representative images at × 400 magnification are shown. c The level of COMP in the LX2 and CM was confirmed by Western blot and ELISA after knockdown by siRNAs. The NC siRNA was used as control. n = three independent repeats. P < 0.05 by t test versus control. d The expression of the indicated proteins in HCC cells after co-cultured with LX2 cells after knockdown of COMP were examined by Western blot. β-actin was used as a loading control. Western blot analysis was independently repeated for three times with similar results. e The proposed model by which HSCs-derived COMP promotes HCC progression by activating MEK/ERK and PI3K/AKT signaling pathway via a CD36-dependent manner. ( *P < 0.05 , **P < 0.01)
Article Snippet: Quantitative measurement of human COMP in serum of HCC patients were performed by
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Negative Control, Marker, Knockdown, Control, Cell Culture